一种山羊支原体山羊肺炎亚种多重抗原肽的小鼠免疫试验

doi:10.11843/j.issn.0366-6964.2016.12.018

Abstract

Abstract:

A multiple antigenic peptide (MAP), composed of six B-cell epitopes and three T-cell epitopes derived from antigenic proteins of Mycoplasma capricolum subsp. capripneumoniae (Mccp), was designed and expressed in Escherichia coli cells, which was then injected into mice to evaluate humoral and cellular immune responses induced by the recombinant MAP. Antibody levels against MAP, each single B-cell epitope, and the ultrasonic-treated crude Mccp antigen were all significantly increased (P<0.01). Results of in vitro metabolic inhibition test indicated that diluted sera (1:64) from immunized mice inhibited Mccp growth. Splenic lymphocytes from the immunized mice exhibited remarkable proliferation when stimulated by each single T-cell epitope and crude Mccp antigen (P<0.01). The production of IFN-γ, TNF-α, IL-1β, and IL-10 were increased (P<0.001), while that of IL-12 was decreased (P<0.001). These data showed that the constructed MAP stimulated immune responses in mice. The results laid a certain foundation to develop subunit vaccine against contagious caprine pleuropneumonia caused by Mccp.

CLC Number:

S852.62

ZHAO Ping, CHEN Sheng-li，HAO Hua-fang, LI Xue-rui, HE Ying, CHU Yue-feng, LIU Yong-sheng. Immune Responses in Mice Immunized with a Recombinant Multiple Antigenic Peptide from Mycoplasma capricolum Subsp. capripneumoniae[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(12): 2476-2482.

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Immune Responses in Mice Immunized with a Recombinant Multiple Antigenic Peptide from Mycoplasma capricolum Subsp. capripneumoniae

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